Disease:

 Breast adenocarcinoma 

Origin:

 Isolated from pleural effusions of a breast cancer patient. Cells exhibit some features of differentiated mammary epithelium including estradiol synthesis and formation of domes. Cells express both the wild type and variant estrogen receptors as well as progesterone receptor. 

Species:

Homo sapiens 

Tissue:

Breast, mammary gland 

Morphology:

Epithelial 

Properties:

Adherent 

Cytogenic data:

2n = 46, hypertriploid to hypotetraploid. 

Patient:

Female, Caucasian, 69 yrs of age 

Medium:

EMEM (EBSS) + 2mM Glutamine + 0.01 mg/ml human recombinant insulin + 10% Foetal Bovine Serum (FBS). 

Subculture:

1:2 to 1:6 (80-90%) using 0.25% trypsin or trypsin/EDTA, 5% CO2; 37 °C 

Freezing Medium:

Complete culture medium supplemented with 5% (v/v) DMSO 

Biosafety Level:

 I 

Sterility:

Bacteria: Negative 

Yeast: Negative 

Mycoplasma: Negative 

Pathogens:

HIV: Negative 

Hepatitis B: Negative 

Hepatitis C: Negative 

Protein Expression:
 estrogen receptor 
Isoenzymes:
 AK-1,1; ES-D, 1-2; G6PD, B; GLO-I, 1-2; PGM1, 1-2; PGM3,1 
DNA Profile (STR):
 Amelogenin: X; CSF1PO:10; D13S317:11; D16S539:11,12; D5S818:11,12; D7S820:8,9; TH01:6; TPOX:9,12; vWA:14,15; 
Mutations in MCF-7 Cells 

Related cells by Organ:

4T1 

JIMT-1 

MDA-MB-231 

SK-BR-3 

T47D 

MCF-7 


References for MCF-7 cells:

1. J Nat Cancer Inst 1973;51:1409.

2. Cancer Res 1993;53:5882.