慢病毒浓缩试剂盒-常用生化试剂-试剂-生物在线

慢病毒浓缩试剂盒

商家询价

产品名称：慢病毒浓缩试剂盒

英文名称： Lentivirus Concentration Solution

产品编号： JY03011

产品价格： 500

产品产地：江苏南京

品牌商标：江苑生物

更新时间： 2025-12-01T09:58:31

使用范围： null

规格	价格
50 ml	500.0
100 ml	900.0

南京江苑生物科技有限公司

联系人 :
地址 : 江北新区智能制造产业园（智合园）科创大道9号A2栋
邮编 : 210000
所在区域 : 江苏
电话 : 177****6632 点击查看
传真 : 点击查看
邮箱 : jiangyuanbio@163.com

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产品详情

产品简介 慢病毒浓缩试剂盒中含有5×浓缩液，能对所有类型的慢病毒颗粒进行简单快速地浓缩。该试剂盒操作简单，仅需将5×浓缩液与慢病毒上清按比例混合并短时间孵育，然后使用普通的离心机离心，即可得到浓缩的慢病毒颗粒，回收率高达90%以上，可提高100倍病毒滴度。 保存条件 常温配送。4 oC保存，1年有效。 注意事项 1．本产品仅用于科学研究，不得用于临床诊断和治疗，不得用于食品和药品，不得存放于普通住宅内。 2．为了您的安全和健康，请穿实验服并戴一次性手套操作。 使用说明 1．收集慢病毒上清液，0.45 μm滤膜过滤，以除去细胞和碎片。 Note：如果使用滤膜过滤，只能使用低蛋白结合力的纤维素醋酸酯或聚醚砜（PES）膜。不能用**纤维素膜（NC）。 2．将慢病毒上清与试剂盒中的浓缩液按照4:1的比例混合（上清液4份，浓缩液1份）4°C 孵育2h或过夜。刚开始时每隔30min混匀一下，进行3次。 Note：延长孵育时间可以提高回收率。慢病毒颗粒在4 °C可以稳定保存数天。 3．于4°C，4000 g 离心25 min。 4．小心去除上清，不要晃动管子，一般可见白色沉淀（有时沉淀不可见）。 5．用初始体积（原上清液体积）1/10 - 1/100的DMEM或PBS重悬病毒颗粒，小心吹打均匀，重悬沉淀物。 6．测定浓缩慢病毒样品的滴度，50 μL/管分装，于-80 °C冰箱保存。 Note：慢病毒冻存液避免反复冻融，否则会降低病毒滴度。  相关产品 <table class="MsoNormalTable" style="margin-left: 5.4pt; border-collapse: collapse; border: none; mso-border-alt: solid windowtext 1.0pt; mso-yfti-tbllook: 1184; mso-padding-alt: 0cm 5.4pt 0cm 5.4pt; mso-border-insideh: 1.0pt solid windowtext; mso-border-insidev: 1.0pt solid windowtext;" border="1" cellspacing="0" cellpadding="0"> <tbody> <tr style="mso-yfti-irow: 0; mso-yfti-firstrow: yes;"> <td style="width: 142.95pt; border: solid windowtext 1.0pt; background: #9CC2E5; padding: 0cm 5.4pt 0cm 5.4pt;" valign="top" width="191"> 产品编号 </td> <td style="width: 163.0pt; border: solid windowtext 1.0pt; border-left: none; mso-border-left-alt: solid windowtext 1.0pt; background: #9CC2E5; padding: 0cm 5.4pt 0cm 5.4pt;" valign="top" width="217"> 产品名称 </td> <td style="width: 127.85pt; border: solid windowtext 1.0pt; border-left: none; mso-border-left-alt: solid windowtext 1.0pt; background: #9CC2E5; padding: 0cm 5.4pt 0cm 5.4pt;" valign="top" width="170"> 产品规格 </td> </tr> <tr style="mso-yfti-irow: 1;"> <td style="width: 142.95pt; border: solid windowtext 1.0pt; border-top: none; mso-border-top-alt: solid windowtext 1.0pt; padding: 0cm 5.4pt 0cm 5.4pt;" valign="top" width="191"> JY03011/JY03012 </td> <td style="width: 163.0pt; border-top: none; border-left: none; border-bottom: solid windowtext 1.0pt; border-right: solid windowtext 1.0pt; mso-border-top-alt: solid windowtext 1.0pt; mso-border-left-alt: solid windowtext 1.0pt; padding: 0cm 5.4pt 0cm 5.4pt;" valign="top" width="217"> 慢病毒浓缩试剂盒 </td> <td style="width: 127.85pt; border-top: none; border-left: none; border-bottom: solid windowtext 1.0pt; border-right: solid windowtext 1.0pt; mso-border-top-alt: solid windowtext 1.0pt; mso-border-left-alt: solid windowtext 1.0pt; padding: 0cm 5.4pt 0cm 5.4pt;" valign="top" width="170"> 50 mL/100 mL </td> </tr> <tr style="mso-yfti-irow: 2;"> <td style="width: 142.95pt; border: solid windowtext 1.0pt; border-top: none; mso-border-top-alt: solid windowtext 1.0pt; padding: 0cm 5.4pt 0cm 5.4pt;" valign="top" width="191"> JY03013/JY03014 </td> <td style="width: 163.0pt; border-top: none; border-left: none; border-bottom: solid windowtext 1.0pt; border-right: solid windowtext 1.0pt; mso-border-top-alt: solid windowtext 1.0pt; mso-border-left-alt: solid windowtext 1.0pt; padding: 0cm 5.4pt 0cm 5.4pt;" valign="top" width="217"> PEG-8000慢病毒浓缩液 </td> <td style="width: 127.85pt; border-top: none; border-left: none; border-bottom: solid windowtext 1.0pt; border-right: solid windowtext 1.0pt; mso-border-top-alt: solid windowtext 1.0pt; mso-border-left-alt: solid windowtext 1.0pt; padding: 0cm 5.4pt 0cm 5.4pt;" valign="top" width="170"> 50 mL/100 mL </td> </tr> <tr style="mso-yfti-irow: 3;"> <td style="width: 142.95pt; border: solid windowtext 1.0pt; border-top: none; mso-border-top-alt: solid windowtext 1.0pt; padding: 0cm 5.4pt 0cm 5.4pt;" valign="top" width="191"> JY03021/JY03022/JY03023 </td> <td style="width: 163.0pt; border-top: none; border-left: none; border-bottom: solid windowtext 1.0pt; border-right: solid windowtext 1.0pt; mso-border-top-alt: solid windowtext 1.0pt; mso-border-left-alt: solid windowtext 1.0pt; padding: 0cm 5.4pt 0cm 5.4pt;" valign="top" width="217"> 慢病毒包装剂盒 </td> <td style="width: 127.85pt; border-top: none; border-left: none; border-bottom: solid windowtext 1.0pt; border-right: solid windowtext 1.0pt; mso-border-top-alt: solid windowtext 1.0pt; mso-border-left-alt: solid windowtext 1.0pt; padding: 0cm 5.4pt 0cm 5.4pt;" valign="top" width="170"> 10T/20T/40T (10cm dish) </td> </tr> <tr style="mso-yfti-irow: 4;"> <td style="width: 142.95pt; border: solid windowtext 1.0pt; border-top: none; mso-border-top-alt: solid windowtext 1.0pt; padding: 0cm 5.4pt 0cm 5.4pt;" valign="top" width="191"> JY03024/JY03025/JY03026 </td> <td style="width: 163.0pt; border-top: none; border-left: none; border-bottom: solid windowtext 1.0pt; border-right: solid windowtext 1.0pt; mso-border-top-alt: solid windowtext 1.0pt; mso-border-left-alt: solid windowtext 1.0pt; padding: 0cm 5.4pt 0cm 5.4pt;" valign="top" width="217"> 慢病毒包装质粒Mix </td> <td style="width: 127.85pt; border-top: none; border-left: none; border-bottom: solid windowtext 1.0pt; border-right: solid windowtext 1.0pt; mso-border-top-alt: solid windowtext 1.0pt; mso-border-left-alt: solid windowtext 1.0pt; padding: 0cm 5.4pt 0cm 5.4pt;" valign="top" width="170"> 100 μg/200 μg/400 μg </td> </tr> <tr style="mso-yfti-irow: 5;"> <td style="width: 142.95pt; border: solid windowtext 1.0pt; border-top: none; mso-border-top-alt: solid windowtext 1.0pt; padding: 0cm 5.4pt 0cm 5.4pt;" valign="top" width="191"> JY03027/JY03028 </td> <td style="width: 163.0pt; border-top: none; border-left: none; border-bottom: solid windowtext 1.0pt; border-right: solid windowtext 1.0pt; mso-border-top-alt: solid windowtext 1.0pt; mso-border-left-alt: solid windowtext 1.0pt; padding: 0cm 5.4pt 0cm 5.4pt;" valign="top" width="217"> 慢病毒包装质粒 pMD2.G </td> <td style="width: 127.85pt; border-top: none; border-left: none; border-bottom: solid windowtext 1.0pt; border-right: solid windowtext 1.0pt; mso-border-top-alt: solid windowtext 1.0pt; mso-border-left-alt: solid windowtext 1.0pt; padding: 0cm 5.4pt 0cm 5.4pt;" valign="top" width="170"> 1 μg/100 μg </td> </tr> <tr style="mso-yfti-irow: 6;"> <td style="width: 142.95pt; border: solid windowtext 1.0pt; border-top: none; mso-border-top-alt: solid windowtext 1.0pt; padding: 0cm 5.4pt 0cm 5.4pt;" valign="top" width="191"> JY03029/JY03030 </td> <td style="width: 163.0pt; border-top: none; border-left: none; border-bottom: solid windowtext 1.0pt; border-right: solid windowtext 1.0pt; mso-border-top-alt: solid windowtext 1.0pt; mso-border-left-alt: solid windowtext 1.0pt; padding: 0cm 5.4pt 0cm 5.4pt;" valign="top" width="217"> 慢病毒包装质粒 psPAX2 </td> <td style="width: 127.85pt; border-top: none; border-left: none; border-bottom: solid windowtext 1.0pt; border-right: solid windowtext 1.0pt; mso-border-top-alt: solid windowtext 1.0pt; mso-border-left-alt: solid windowtext 1.0pt; padding: 0cm 5.4pt 0cm 5.4pt;" valign="top" width="170"> 1 μg/100 μg </td> </tr> <tr style="mso-yfti-irow: 7;"> <td style="width: 142.95pt; border: solid windowtext 1.0pt; border-top: none; mso-border-top-alt: solid windowtext 1.0pt; padding: 0cm 5.4pt 0cm 5.4pt;" valign="top" width="191"> JY03031/JY03032 </td> <td style="width: 163.0pt; border-top: none; border-left: none; border-bottom: solid windowtext 1.0pt; border-right: solid windowtext 1.0pt; mso-border-top-alt: solid windowtext 1.0pt; mso-border-left-alt: solid windowtext 1.0pt; padding: 0cm 5.4pt 0cm 5.4pt;" valign="top" width="217"> 慢病毒包装质粒 pCMV-VSV-G </td> <td style="width: 127.85pt; border-top: none; border-left: none; border-bottom: solid windowtext 1.0pt; border-right: solid windowtext 1.0pt; mso-border-top-alt: solid windowtext 1.0pt; mso-border-left-alt: solid windowtext 1.0pt; padding: 0cm 5.4pt 0cm 5.4pt;" valign="top" width="170"> 1 μg/100 μg </td> </tr> <tr style="mso-yfti-irow: 8;"> <td style="width: 142.95pt; border: solid windowtext 1.0pt; border-top: none; mso-border-top-alt: solid windowtext 1.0pt; padding: 0cm 5.4pt 0cm 5.4pt;" valign="top" width="191"> JY03033/JY03034 </td> <td style="width: 163.0pt; border-top: none; border-left: none; border-bottom: solid windowtext 1.0pt; border-right: solid windowtext 1.0pt; mso-border-top-alt: solid windowtext 1.0pt; mso-border-left-alt: solid windowtext 1.0pt; padding: 0cm 5.4pt 0cm 5.4pt;" valign="top" width="217"> 慢病毒包装质粒 pCMV-dR8.91 </td> <td style="width: 127.85pt; border-top: none; border-left: none; border-bottom: solid windowtext 1.0pt; border-right: solid windowtext 1.0pt; mso-border-top-alt: solid windowtext 1.0pt; mso-border-left-alt: solid windowtext 1.0pt; padding: 0cm 5.4pt 0cm 5.4pt;" valign="top" width="170"> 1 μg/100 μg </td> </tr> <tr style="mso-yfti-irow: 9;"> <td style="width: 142.95pt; border: solid windowtext 1.0pt; border-top: none; mso-border-top-alt: solid windowtext 1.0pt; padding: 0cm 5.4pt 0cm 5.4pt;" valign="top" width="191"> JY03035/JY03036 </td> <td style="width: 163.0pt; border-top: none; border-left: none; border-bottom: solid windowtext 1.0pt; border-right: solid windowtext 1.0pt; mso-border-top-alt: solid windowtext 1.0pt; mso-border-left-alt: solid windowtext 1.0pt; padding: 0cm 5.4pt 0cm 5.4pt;" valign="top" width="217"> 慢病毒包装质粒 pMDLg/pRRE </td> <td style="width: 127.85pt; border-top: none; border-left: none; border-bottom: solid windowtext 1.0pt; border-right: solid windowtext 1.0pt; mso-border-top-alt: solid windowtext 1.0pt; mso-border-left-alt: solid windowtext 1.0pt; padding: 0cm 5.4pt 0cm 5.4pt;" valign="top" width="170"> 1 μg/100 μg </td> </tr> <tr style="mso-yfti-irow: 10; mso-yfti-lastrow: yes;"> <td style="width: 142.95pt; border: solid windowtext 1.0pt; border-top: none; mso-border-top-alt: solid windowtext 1.0pt; padding: 0cm 5.4pt 0cm 5.4pt;" valign="top" width="191"> JY03037/JY03038 </td> <td style="width: 163.0pt; border-top: none; border-left: none; border-bottom: solid windowtext 1.0pt; border-right: solid windowtext 1.0pt; mso-border-top-alt: solid windowtext 1.0pt; mso-border-left-alt: solid windowtext 1.0pt; padding: 0cm 5.4pt 0cm 5.4pt;" valign="top" width="217"> 慢病毒包装质粒 pRSV-Rev </td> <td style="width: 127.85pt; border-top: none; border-left: none; border-bottom: solid windowtext 1.0pt; border-right: solid windowtext 1.0pt; mso-border-top-alt: solid windowtext 1.0pt; mso-border-left-alt: solid windowtext 1.0pt; padding: 0cm 5.4pt 0cm 5.4pt;" valign="top" width="170"> 1 μg/100 μg </td> </tr> </tbody> </table>