人真皮成纤维细胞(原代细胞永生化)
D/F12+10%FBS+1%p/s+0.005mg/ml胰岛素+5ng/mlBfGF+1ug/ml氢化可的松+50ug/ml抗坏血酸(维C)+7.5mM L-Gln (推荐:PriMed-iCell-003)
人胰腺导管细胞
75%DMEM(无糖)+25%Medium M3 +5%FBS+10ng/mlrh EGF+2.5mM D-葡萄糖+750 ng/ml 嘌呤霉素+1%P/S
人真皮成纤维细胞(原代细胞永生化)
D/F12+10%FBS+1%p/s+0.005mg/ml胰岛素+5ng/mlBfGF+1ug/ml氢化可的松+50ug/ml抗坏血酸(维C)+7.5mM L-Gln (推荐:PriMed-iCell-003)
人胰腺导管细胞
75%DMEM(无糖)+25%Medium M3 +5%FBS+10ng/mlrh EGF+2.5mM D-葡萄糖+750 ng/ml 嘌呤霉素+1%P/S